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NAD+ accepts electrons and hydrogen atoms to become NADH during several key processes: Glycolysis, where each glucose molecule generates 2 molecules of NADH [7] The tricarboxylic acid (TCA) cycle, which converts 4 molecules of NAD+ to NADH using one molecule of pyruvate [8] Fatty acid β-oxidation, where NAD+ helps break down fats for energy [8] Mitochondrial electron transport chain, where NADH donates electrons to produce ATP [9] The cell's energy status is reflected in its NAD+/NADH ratio