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Finally, sections were incubated, covered, with fluorescent secondary antibodies at a 1:200 dilution in blocking solution at room temperature on a shaker for 1 hr, followed by 3 washes in 1X PBS prior to mounting on microscope slides and coverslipping with Fluoromount-G with or without DAPI (0100–20 and 0100–01

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Think of BPC-157 as one input instead of a stand-in for the basics