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Specifically, protein activity in malignant cells was assessed using metaVIPER 140 by integrating six PAAD gene regulatory networks, which we generated independently from distinct PAAD cohorts, including single-cell profiles (scNET) 43 , laser microdissected samples (CUMC-net) 164 , the TCGA PAAD cohort (TCGA-net) 165 , the ICGC PAAD cohort (ICGC-net) 166 , the UNC cohort (UNC-net) 163 and single-cell profiles from HCMI samples (HCMI-net)