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3.2 Chapter Specific Learning Outcomes Upon successful completion of this chapter, you will be able to: Describe the structure of GPCRs Explain how the structural properties dictate isolation of GPCRs Distinguish between two classes of G proteins Describe the subunit composition of heterotrimeric G proteins Demonstrate how the Gα subunit functions analogous to a molecular switch Outline the general steps in the GPCR signalling pathway Differentiate between first and second messenger, providing an example for each Illustrate downstream effects in the GPCR pathway List the reaction catalyzed by Adenylyl Cyclase (AC) Describe how the enzyme AC can regulate gene expression Interpret signalling pathways 3.3 Structural and Functional Properties of GPCRs 3.3.1 Describe the canonical structure of GPCRs