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Materials and Methods Fluorescent instrumentation Perkin-Elmer fluorescent spectrometer model 1000 set at excitation wavelength 475nm and its scale expansion gain control knob was adjusted so that a 10μM solution of the bimane conjugate gave a full scale reading of 100 fluorescence units

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The remaining interference mechanisms, singlet oxygen and light quenchers as well as light scatterers are identified by incubating the hit compounds with beads that have been premixed