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Take the shears or hoof knife and trim the heel, sole and walls to form a flat surface

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NMR spectroscopy is regarded as one of the most reliable methods to study lipid peroxidation for two reasons: (i) it can investigate changes in the starting materials as well as the evolution of various oxidation products during the process, and (ii) does not require any chemical reaction, contrary to various spectrophotometric techniques [26,28,29]

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