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Total flavonoid content was determined using the sodium nitrite-aluminum nitrate colorimetric method and measured by UV spectrophotometry, with results expressed as μg·g −1 FW ( Table 3 presents the calibration data and analytical parameters for kaempferol, quercetin, and total flavonoids, including the standard curve equations, R 1 values, recovery rates, and limits of detection (LOD) and quantification (LOQ)

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The substrate-induced CTPS1 polymerization results in a domain rotation that aligns the two active sites, creating openings at both active sites that can potentially facilitate ammonia relocation, although the complete tunnel architecture remains to be identified

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