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The fact that increased xCT activity can substantially increase intracellular CYS abundance and thereby promote reactions with endogenous biomolecules suggests that alterations to CYS levels may impact cell function, so we investigated the functional consequences of increased CYS 2 uptake on NRF2 on bile duct cell lines with high SLC7A11 expression (SNU308 and TFK1) versus NRF2 off bile duct cell lines with low SLC7A11 expression (CCLP1 and YSCCC)