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Additionally, we wish to introduce one study that is not in the database that may help achieve the overall goal of our review

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The resin, washed with lysis buffer, was treated with thrombin digestion buffer (50 mM Tris–HCl, pH 8.0, 150 mM NaCl, and 2.5 mM CaCl 2 ) containing 3.5 unit/ml bovine thrombin for 15 min at 25 °C