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NAC in very high doses (above 1800mg daily) can theoretically affect cysteine metabolism and insulin signalling, which may be relevant for diabetic patients on tirzepatide, but this is uncommon at standard supplementation levels

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Freshly prepared TGM2 was assayed with 10 ng protein/well, whereas 40 ng protein/well was used with enzyme stored at 5 ⁰C for 6 days