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Organoids provide both a unique and powerful model to interrogate disease, understand regeneration and potentially provide the building blocks for bridging therapies for patients waiting for an organ or ultimately provide a replacement organ
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Proteins were separated by SDS–PAGE, transferred to PVDF membranes (Millipore) and probed with primary antibodies in 1× TBST buffer (20 mM Tris pH 7.5, 150 mM NaCl and 0.1% Tween-20) with 5% fat-free milk at 4 °C overnight
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