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More evidence for the reduction of the pro-inflammatory response by EGb 761 is the inhibition of microglial inflammatory responses using BV2 microglial cell lines, which, assessed by qPCR, showed a significant increase in pro-inflammatory cytokines TNFα, IL-1β and IL-6 compared to control cells

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The ratio of dihexa/inulin in blood was then used to account for any blood contamination in the various brain regions