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Comparisons between methods (1) and (5) yielded conflicting results: some studies reported no significant differences in repigmentation rate or regeneration size [148], whereas others reported higher repigmentation rates with method (1) [149]

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Methods Cell lines and reagents HEK293T and HeLa cells were maintained in high-glucose DMEM (Sigma-Aldrich, D6429) containing 10% fetal calf serum (Sigma-Aldrich, P4333), 100 U ml −1 penicillin and 100 µg ml −1 streptomycin at 37 °C and 5% CO 2

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