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E2E1-I53-50A trimers assemble into nanoparticles efficiently To assemble E2E1-I53-50A into nanoparticles (E2E1-NP), we mixed the purified E2E1-I53-50A trimers with the pentameric I53-50B.4PT1 and we used SEC to separate the expected higher molecular weight nanoparticles from the E2E1-I53-50A trimers 46 (Fig