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更具体地,按照本发明,依次连接具有保护基团的氨基酸,获得保护BPC157肽树脂,其间依次脱去Fmoc保护基团的方法包括以下步骤: More specifically, according to the present invention, the amino acids with protective groups are sequentially connected to obtain the protected BPC157 peptide resin, and the method for sequentially removing the Fmoc protective group includes the following steps: (1)制备Fmoe-Val-树脂: (1) Preparation of Fmoe-Val-resin: 选用三苯氯甲基树脂、4-甲基-三苯氯甲基树脂、4-甲氧基-三苯氯甲基树脂、2-氯-三苯氯甲基树脂或王氏树脂中任一种树脂,用N,N-二甲基甲酰胺或二氯甲烷浸泡10~60分钟,混合物中,树脂的重量体积浓度为5-20ml/g;然后在上述的树脂中,加入DIEA、DIC或DMAP、Fmoc-Val-OH,10~50℃反应0.5~5小时,再加入封闭试剂甲醇、苯甲酰氯或吡啶10~50℃反应0.2-3小时,树脂用异丙醇、N,N-二甲基甲酰胺洗涤,获得Fmoc-Val-树脂; Choose any of trityl chloromethyl resin, 4-methyl-trityl chloromethyl resin, 4-methoxy-trityl chloromethyl resin, 2-chloro-trityl chloromethyl resin or Wang's resin A kind of resin is soaked with N,N-dimethylformamide or dichloromethane for 10-60 minutes