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In brief, about 10 mg frozen tissue per sample were homogenized under denaturing conditions with a FastPrep (2 times for 60 s, 4 m x s-1) in 1 ml of a buffer containing 6 M guanidinium chloride (GdmCl), 5 mM tris(2-carboxyethyl)phosphine, 20 mM chloroacetamide and 50 mM Tris-HCl pH 8.5