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However, the more pronounced differences in astrocyte reactivity, microglial activation, oxidative stress, tissue swelling and demyelination within the cuprizone pellet group compared to their controls at 3 weeks, despite no significant weight loss, suggest that weight loss may not be a reliable proxy of the extent of demyelinating disease
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In addition in severe B12 deficiency, the reduced production of free GSH, leads to lower levels of production of iron-sulphur proteins, such as aconitase, thereby leading to lower energy transfer into the Citric Acid Cycle