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ζ΄ε
·δ½ε°οΌζη
§ζ¬εζοΌδΎζ¬‘θΏζ₯ε
·ζδΏζ€εΊε’ηζ°¨εΊι
ΈοΌθ·εΎδΏζ€BPC157θ½ζ θοΌε
Άι΄δΎζ¬‘θ±ε»FmocδΏζ€εΊε’ηζΉζ³ε
ζ¬δ»₯δΈζ₯ιͺ€οΌ More specifically, according to the present invention, the amino acids with protective groups are sequentially connected to obtain the protected BPC157 peptide resin, and the method for sequentially removing the Fmoc protective group includes the following steps: (1)εΆε€Fmoe-Val-ζ θοΌ (1) Preparation of Fmoe-Val-resin: ιη¨δΈθ―ζ°―η²εΊζ θγ4-η²εΊ-δΈθ―ζ°―η²εΊζ θγ4-η²ζ°§εΊ-δΈθ―ζ°―η²εΊζ θγ2-ζ°―-δΈθ―ζ°―η²εΊζ θζηζ°ζ θδΈδ»»δΈη§ζ θοΌη¨NοΌN-δΊη²εΊη²ι
°θΊζδΊζ°―η²η·ζ΅Έζ³‘10ο½60ειοΌζ··εη©δΈοΌζ θηιιδ½η§―ζ΅εΊ¦δΈΊ5-20ml/gοΌηΆεε¨δΈθΏ°ηζ θδΈοΌε ε
₯DIEAγDICζDMAPγFmoc-Val-OHοΌ10ο½50βεεΊ0.5ο½5ε°ζΆοΌεε ε
₯ε°ιθ―εη²ιγθ―η²ι
°ζ°―ζε‘εΆ10ο½50βεεΊ0.2-3ε°ζΆοΌζ θη¨εΌδΈιγNοΌN-δΊη²εΊη²ι
°θΊζ΄ζΆ€οΌθ·εΎFmoc-Val-ζ θοΌ Choose any of trityl chloromethyl resin, 4-methyl-trityl chloromethyl resin, 4-methoxy-trityl chloromethyl resin, 2-chloro-trityl chloromethyl resin or Wang's resin A kind of resin is soaked with N,N-dimethylformamide or dichloromethane for 10-60 minutes