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ζ›΄ε…·δ½“εœ°οΌŒζŒ‰η…§ζœ¬ε‘ζ˜ŽοΌŒδΎζ¬‘θΏžζŽ₯ε…·ζœ‰δΏζŠ€εŸΊε›’ηš„ζ°¨εŸΊι…ΈοΌŒθŽ·εΎ—δΏζŠ€BPC157θ‚½ζ ‘θ„‚οΌŒε…Άι—΄δΎζ¬‘θ„±εŽ»FmocδΏζŠ€εŸΊε›’ηš„ζ–Ήζ³•εŒ…ζ‹¬δ»₯δΈ‹ζ­₯ιͺ€οΌš More specifically, according to the present invention, the amino acids with protective groups are sequentially connected to obtain the protected BPC157 peptide resin, and the method for sequentially removing the Fmoc protective group includes the following steps: (1)εˆΆε€‡Fmoe-Val-ζ ‘θ„‚οΌš (1) Preparation of Fmoe-Val-resin: ι€‰η”¨δΈ‰θ‹―ζ°―η”²εŸΊζ ‘θ„‚γ€4-η”²εŸΊ-δΈ‰θ‹―ζ°―η”²εŸΊζ ‘θ„‚γ€4-η”²ζ°§εŸΊ-δΈ‰θ‹―ζ°―η”²εŸΊζ ‘θ„‚γ€2-ζ°―-δΈ‰θ‹―ζ°―η”²εŸΊζ ‘θ„‚ζˆ–ηŽ‹ζ°ζ ‘θ„‚δΈ­δ»»δΈ€η§ζ ‘θ„‚οΌŒη”¨N,N-δΊŒη”²εŸΊη”²ι…°θƒΊζˆ–δΊŒζ°―η”²ηƒ·ζ΅Έζ³‘10~60εˆ†ι’ŸοΌŒζ··εˆη‰©δΈ­οΌŒζ ‘θ„‚ηš„ι‡ι‡δ½“η§―ζ΅“εΊ¦δΈΊ5-20ml/gοΌ›η„ΆεŽεœ¨δΈŠθΏ°ηš„ζ ‘θ„‚δΈ­οΌŒεŠ ε…₯DIEA、DICζˆ–DMAP、Fmoc-Val-OH,10~50℃反应0.5~5ε°ζ—ΆοΌŒε†εŠ ε…₯ε°ι—­θ―•ε‰‚η”²ι†‡γ€θ‹―η”²ι…°ζ°―ζˆ–ε‘ε•Ά10~50℃反应0.2-3ε°ζ—ΆοΌŒζ ‘θ„‚η”¨εΌ‚δΈ™ι†‡γ€N,N-δΊŒη”²εŸΊη”²ι…°θƒΊζ΄—ζΆ€οΌŒθŽ·εΎ—Fmoc-Val-ζ ‘θ„‚οΌ› Choose any of trityl chloromethyl resin, 4-methyl-trityl chloromethyl resin, 4-methoxy-trityl chloromethyl resin, 2-chloro-trityl chloromethyl resin or Wang's resin A kind of resin is soaked with N,N-dimethylformamide or dichloromethane for 10-60 minutes

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