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The protein was subjected to crystallization screening and was found to crystallize using a Hampton Research Crystal Screen, condition 18 (0.1 M sodium cacodylate pH 6.5, 0.2 M magnesium acetate tetrahydrate, 20% PEG 8000)

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After centrifugation, the NPCs were incubated for 20 min at 37 °C with the following fluorescent probes: Live/Dead calcein violet-AM (0.9 µM, Invitrogen # C34858), tetramethylrhodamine ethyl ester (TMRE)-Mitochondrial Membrane Potential (TMRE