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To investigate which phosphorylation site(s) played a major role, we mutated Y249/Y322 singly into Y249F or Y322 F (one residue to Phe), or doubly into 2YF or 2YA (both residues to Phe or Ala)

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Cross-talk between σ B and PrfA ensures adequate gene expression both inside and outside the host (Gaballa et al., 2019)

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