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Cancer cells exhibit dysregulation of the proteins/enzymes involved in the key regulatory steps of glucose transport, glycolysis, tricarboxylic acid (TCA) cycle and glutaminolysis, governed not only by oncogenes such as c-Myc but also by hypoxia-inducible factor-1 and loss of function tumor suppressor p53 (5)

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The single most important rule: reconstitute only what you plan to use within the labeled stability window, and never freeze the reconstituted solution under any circumstances

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