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The LNs were then extensively washed in a phosphate buffer, snap-frozen, cut into 10-μm sections, and stained with 10 nm gold-conjugated goat anti–rat IgG (EM.GAT10, BBI Solutions) in a phosphate buffer containing 0.5 % fish gelatin and 0.5 % BSA for 3 hours at room temperature

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[DOI] [PMC free article] [PubMed] [Google Scholar] 105.Kalhan SC, Edmison J, Marczewski S, Dasarathy S, Gruca LL, Bennett C, Duenas C, Lopez R