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Sakai introduced specific siRNAs against catalase, GPX4, superoxide dismutase 1 (SOD1), and SOD2 into HCECs (150) and found that lactate dehydrogenase (LDH) release was significantly elevated in the GPX4-, SOD1-, SOD2-, and CAT-knockdown groups, with the greatest increase observed in the GPX4-knockdown group compared with the SOD1-knockdown group

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The main rat strains previously used are Wistar and Sprague-Dawley rats however, Fisher 344 rats have also been used