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Nephrin sheds off from the podocytes and is discharged into the urine when the binding of skeletal protein to the nephrin protein on the surface of podocytes is compromised (Xu et al., 2020)

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Phosphorylated proteins were resolved on 6-10% SDS–PAGE gels and visualized by autoradiography using a Typhoon 9210 imager (GE Healthcare Life Sciences)