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The phage library was created with degenerate primers incorporating NNK triplet nucleotide sequences, which randomly encoded only one amber codon and other sense codes instead of the amber codon only at fixed position 373 in peptide-coding sequences, followed by a superinfection-immunity-based selection to isolate clones containing an amber codon, effectively excluding those with solely sense codons or harmful mutations from the library

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collection for artificial insemination or laboratory evaluation or examination in a clinicopathological sense