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Paneth cells reside in the base of crypts and secrete anti-microbial peptides, such as α-defensin, to impede microbial entry to the intestinal lumen

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In recent years, the metabolomic research ‘toolkit’ has expanded significantly, introducing single-cell metabolic profiling techniques, spatial metabolomics to map the distribution of metabolites in tissues, and systems biology approaches that integrate (single-cell) epigenetic, transcriptomic, and metabolomic datasets