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Here, we describe the GST-His method as a new small-scale affinity purification system for recombinant proteins, based on a N-terminal Glutathione Sepharose Tag (GST) 2,3 and a C-terminal 10xHis tag 4 , which are both fused to the protein of interest

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Accordingly, an important requirement for Desat1 was previously demonstrated for the autophagy pathway in Drosophila 32