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Figure 2 Density functional theory prediction on pyocyanin-ascorbic acid interactions Density functional theory (DFT) predicted two feasible H-bonding interactions between (i) the –CH 2 OH unit of ascorbic acid with –C=O of pyocyanin and (ii) the most acidic –OH unit of ascorbic acid with –C=O of pyocyanin (Figure 3)

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