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Subsequent studies revealed that CSCs, defined by both distinct surface markers and tumor-initiating capabilities, were identified across various cancers, including breast cancer, 27 GBM, 28,29 lung cancer, 30 prostate cancer, 31 colon cancer, 32 head and neck squamous cell carcinoma, 33 pancreatic cancer, 34 and melanoma, 35 further validating the broad applicability of the CSC model
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This innovative approach involved the intramuscular electroporation of plasmids encoding both mouse and human ROS1 in transgenic mice with K-Ras-driven lung adenocarcinomas