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In summary, a large body of experimental evidence accumulated over the past 15 years indicates that peroxynitrite generation from NO and O 2 •− represents a major threat in the cellular environment

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After 96 h, the cells were harvested using pancreatic enzymes, washed twice with PBS, and lysed with 1% TritonX-100 (300 μL for 0 μM CGA and 150 μL for 600 μM CGA) on ice for 10 min

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