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Vitamin B12 status in infancy and the effect of a vitamin B12 injection in infants with subclinical vitamin B12 deficiency: study protocol for a register-based randomised controlled trial | BMJ Open

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10Lμl of SLC33A1 was mixed with equal volumes of two-fold serially diluted solutions of various molecules (6000 μM to 0.732 μM) to achieve a final protein concentration of 1.33 μM of SLC33A1, followed by room temperature incubation for 20 min