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These therapeutics are focused on the patient’s behavior, aiming at symptomatic management instead of modifying or supplementing the affected neuronal pathways [84-88]
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In brief, labeled peptide was diluted to 1 μM in artificial hemolymph and added at a 1:10 ratio to the mounted tissues (final concentration 100 nM), and bound peptide was imaged using an LSM-900 confocal microscope (Zeiss) in AiryScan2 super-resolution mode with a 40x objective and GFP and BODIPY-543 filter settings
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