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Determine the Concentrations and Molecular Weight of Recombinant GHK Peptide by HPLC The retention time and concentrations of the recombinant GHK peptide and GHK standard (Sigma-Aldrich, Darmstadt, Germany) were determined by reverse-phase (Mightysil, RP-18, GP 250-4.6, 5 mm, Kanto Chemical Co., Inc., Tokyo, Japan) high-pressure liquid chromatography (Hitachi High-Performance Liquid Chromatograph Chromaster, Hitachi High-Technologies Corporation, Tokyo, Japan) analysis
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