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DAB staining and preparation of cultured cells for EM The fixative was removed and cells were washed (five times for 1 min) with 0.1 M sodium cacodylate (Ted Pella, 18851) buffer at pH 7.4 containing 2 mM CaCl 2 on ice and blocked with 20 mM glycine in 0.1 M sodium cacodylate buffer pH 7.4 containing 2 mM CaCl 2 for 20 min on ice

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Their controllable, targeted therapeutic effects outperform probiotics and prebiotics in cardiovascular disease intervention while also mitigating the issue of individual response heterogeneity in microbiota therapy ( As a special medical method, engineered bacteria can be used not only for diagnosing and detecting partial intestinal inflammation with their unique sensors (Table 1