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Small-interfering RNA transfection Transfections of siRNAs (a final concentration of 40 nM, in the case of double depletion 20 nM each) were done using Lipofectamine RNAiMAX (Thermo Fisher Scientific, 13778150) according to the instructions of manufacturer (reverse transfection protocol)

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These encompass glycolytic activity, the process of fatty acid β-oxidation (FAO), lipid synthesis, and amino acid cycling