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In parallel, using the algorithm of ShotgunFunctionalizeR, we again identified that vitamin B12 biosynthesis pathway was significantly down-regulated ( P =9.3E-51, ShotgunFunctionalizeR)

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gonorrhea , Chlamydia trachomatis , and Treponema pallidum are common bacterial STIs that can carry antimicrobial resistance genes and are typically not a major component of the vaginal microbiome