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The top layer was aspirated without perturbing the disk, additional MeOH (400 µl) was added, and the proteins were pelleted (10,000 g, 10 min, 4°C)

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[DOI] [PMC free article] [PubMed] [Google Scholar] 98.Hatch M & Freel RW A human strain of Oxalobacter (HC-1) promotes enteric oxalate secretion in the small intestine of mice and reduces urinary oxalate excretion