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A rat model of transient cerebral ischemia was established using MCAO, a widely used experimental model that mimics human ischemic stroke ( -7 g/kg at two time points: 15 minutes before the induction of ischemia and again at the onset of reperfusion, which occurred 2 hours after arterial occlusion ( Among the mechanisms contributing to brain damage after stroke, MMP−9 has been identified as an important mediator of neuronal injury during the reperfusion phase