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Over-the-counter copper peptide products are typically cosmetic-grade, much lower in concentration, and mass-produced to have a shelf life of about a year

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the reaction was initiated by adding 4 ⁇ L of 25 ⁇ M stock purified NNMT protein (final concentration of NNMT in the reaction was 200 nM) and incubated on a heat block at 37° C