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The AlphaFold structure was submitted to the Protein Preparation Workflow on Maestro, by Schrodinger (Suite 2024-4) , to reassign bond orders using CCD (Chemical Component Dictionary) database, add/replace hydrogens, fill in missing side chains, generate disulfide bonds, optimize hydrogen bonds with PROPKA , and perform restrained minimization to 0.30 Å root mean-square deviation (RMSD) using the OPLS4 force field
Adipogenesis Research
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